2013 Fiscal Year Final Research Report
Genetic and Epigenetic Landscape of Endothelial Cells Differentiation Reveal the Transcriptional Factors Network.
| Project/Area Number |
24710227
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
System genome science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KANKI Yasuharu 東京大学, アイソトープ総合センター, 助教 (00534869)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | エピゲノム制御 / 血管内皮細胞 / 細胞分化 / 転写因子 |
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| Research Abstract |
In this report, our aim is to establish the more efficient and practical endothelial cell differentiation system from embryonic stem (ES) or induced pluripotent stem (iPS) cells. On this purpose, we analyzed the endothelial cell differentiation system from mouse ES cells by using two comprehensive analyses ChIP-seqs and micorarrays.
From these experiments we found out H3K4me3 have changed on early stage during differentiation. Moreover, we discovered some bivalent genes (H3K4me3 and H3K27me3 double positive) such as Etv2, Gata2, Sox18, Sox7 and Fli1 which are upregulated at early time points and supposed to be candidates for master regulators of endothelial cells differentiation. Knockdown of these factors caused drastic reduction of endothelial cell differentiation efficiency.
Taken together, these findings have suggested that determination of cell fate is based on not only master transcription factors but also epigenetic histone modifications.
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[Journal Article] GATA factor switching from GATA2 to GATA1 contributes to erythroid differentiation2013
Author(s)
Suzuki M, Kobayashi-Osaki M, Tsutsumi S, Pan X, Ohmori S, Takai J, Moriguchi T, Ohneda O, Ohneda K, Shimizu R, Kanki Y, Kodama T, Aburatani H, Yamamoto M
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Journal Title
Genes Cells
Volume: 18(11)
Pages: 921-933
DOI
Peer Reviewed
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[Journal Article] Inhibition of histone demethylase JMJD1A improves anti-angiogenic therapy and reduces tumor-associated macrophages2013
Author(s)
Osawa T, Tsuchida R, Muramatsu M, Shimamura T, Wang F, Suehiro J, Kanki Y, Wada Y, Yuasa Y, Aburatani H, Miyano S, Minami T, Kodama T, Shibuya M
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Journal Title
Cancer Res
Volume: 73(10)
Pages: 3019-3028
DOI
Peer Reviewed
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[Journal Article] TNFαsignals through specialized factories where responsive coding and miRNA genes are transcribed2012
Author(s)
Papantonis A, Kohro T, Baboo S, Larkin JD, Deng B, Short P, Tsutsumi S, Taylor S, Kanki Y, Kobayashi M, Li G, Poh HM, Ruan X, Aburatani H, Ruan Y, Kodama T, Wada Y
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Journal Title
EMBO J
Volume: 31(23)
Pages: 4404-4414
DOI
Peer Reviewed
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[Journal Article] Dynamic change of chromatin conformation in response to hypoxia enhances the expression of GLUT3 (SLC2A3) by cooperative interaction of hypoxia-inducible factor 1 and KDM3A2012
Author(s)
Mimura I, Nangaku M, Kanki Y, Tsutsumi S, Inoue T, Kohro T, Yamamoto S, Fujita T, Shimamura T, Suehiro J, Taguchi A, Kobayashi M, Tanimura K, Inagaki T, Tanaka T, Hamakubo T, Sakai J, Aburatani H, Kodama T, Wada Y
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Journal Title
Mol Cell Biol
Volume: 32(15)
Pages: 3018-3032
DOI
Peer Reviewed
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[Journal Article] Alternative splicing of CD44 mRNA by ESRP1 enhances lung colonization of metastatic cancer cell2012
Author(s)
Yae T, Tsuchihashi K, Ishimoto T, Motohara T, Yoshikawa M, Yoshida GJ, Wada T, Masuko T, Mogushi K, Tanaka H, Osawa T, Kanki Y, Minami T, Aburatani H, Ohmura M, Kubo A, Suematsu M, Takahashi K, Saya H, Nagano O
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Journal Title
Nat Commun
Volume: 3
Pages: 883
DOI
Peer Reviewed
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