2013 Fiscal Year Final Research Report
Analysis of bacteria in a fluctuating environment at the single-cell level
| Project/Area Number |
24710228
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
System genome science
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| Research Institution | Osaka University |
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Principal Investigator |
TSURU Saburo 大阪大学, 情報科学研究科, 助教 (80594506)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 一細胞観察 / 遺伝子発現の確率性 / マイクロ流路 |
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| Research Abstract |
We tried to construct a tiny chamber which enables rapid medium replacement under microscope to observe living cells at the single-cell level in real time. This micro chamber consists two parts: a small room for cell growth without washout from the fluid; a channel providing various concentrations of medium to the room by oscillating flow speeds of multiple inlets. Using fluorescent beads and pigment in the medium, we measured the speed of medium replacement in the room. As a result, the chamber replaced the medium in the room within 10 sec, less than half time of our expectation. Subsequently, the bacterial cells producing fluorescence proteins were loaded to the chamber and proliferated in the room by feeding the fresh medium. The protein concentrations fluctuated overtime accompanied with cell growth and varied among isogenic sister cells as a consequence. Thus, the micro chamber with rapid medium replacement revealed the cellular individuality even in the constant environment.
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