Genetic diveristy and ecological adaptation mechanism in Vicia sativa revealed by genomic analysis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24710237
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied genomics
• Research Institution: Kazusa DNA Research Institute
• Principal Investigator: SHIRASAWA Kenta 公益財団法人かずさDNA研究所, 先端研究部, 研究員 (60527026)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ヤハズエンドウ / ゲノム / RAD-Seq法 / 多様性解析

Outline of Final Research Achievements

With the aim of understanding genetic diversity of wild weeds adapted environments, genomics study was carried out on Vicia sativa as a model.
The genetic diversity of populations collected from a local area in Chiba, Japan was revealed with SSR maker analysis. To enhance this analysis broadly, draft genome assembly of Vicia sativa was established with next-generation sequencing technologies. Simultaneously, transcriptome data was also obtained from 10 organs including leaves, stems and roots. These information contributed to detect >10,000 high-confident SNPs among divergent lines of Vicia sativa from inside and outside Japan.
We concluded that regional ecotypes of Vicia sativa have independently maintained their genetic diversity in accordance with the regions, by which the ecotypes are locally adapted to each environment.

Free Research Field

植物遺伝育種学