2013 Fiscal Year Final Research Report
Structural elucidation of molecular basis of cytostatic activity of a novel peptide from a wild silkworm and its application
| Project/Area Number |
24710240
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Living organism molecular science
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| Research Institution | Hokkaido University |
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Principal Investigator |
KAMIYA Masakatsu 北海道大学, 先端生命科学研究科(研究院), 助教 (30399810)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 細胞増殖抑制活性 / ペプチド / 昆虫 / NMR |
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| Research Abstract |
Yamamarin, a novel insect pentapeptide with an amidated C-terminus, has been isolated from diapausing pharate first-instar larvae of the wild silkmoth and is thought to be responsible for the regulation of diapause. Recently, this peptide significantly suppresses the proliferation of rat hepatoma (liver cancer) cells. This finding strongly suggests that yamamarin and its derivatives are promising candidates for use as therapeutic agents. However, its action mechanisms remain unknown. In this study, we investigated the interaction between yamamarin and fibrillarin. The following results were obtained.
(1) We have successfully obtained a recombinant fibrillarin using E. coli expression system. (2) The CD results indicated that a recombinant fibrillarin was correctly folded. (3) The docking calculation suggested that yamamarin can bind to a substrate-binding site of fibrillarin.
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Research Products
(13 results)
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[Journal Article] Interaction between tachyplesin I, an antimicrobial peptide derived from horseshoe crab, and lipopolysaccharide2014
Author(s)
Kushibiki T., Kamiya M., Aizawa T., Kumaki Y., Kikukawa T., Mizuguchi M., Demura M., Kawabata S.-I., Kawano K.
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Journal Title
Biochim. Biophys. Acta
Volume: 1844
Pages: 527-534
DOI
Peer Reviewed
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[Journal Article] Overexpression of an antimicrobial peptide derived from C. elegans using an aggregation-prone protein coexpression system2013
Author(s)
Tomisawa S., Hojo E., Umetsu Y., Ohki S., Kato Y., Miyazawa M., Mizuguchi M., Kamiya M., Kumaki Y., Kikukawa T., Kawano K., Demura M., Aizawa T.
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Journal Title
AMB Express
Volume: 3
Pages: 45-52
DOI
Peer Reviewed
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