Research on the target identification and mechanism analysis of low molecular weight compound

2013 Fiscal Year Final Research Report

• Project/Area Number: 24710244
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Living organism molecular science
• Research Institution: University of Tsukuba
• Principal Investigator: NAGUMO YOKO 筑波大学, 生命環境系, 助教 (70373339)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 分子標的探索 / 生理活性物質 / 生物分子科学

Research Abstract

The target identification and mechanism validation of a small compound is important but difficult step. To improve methods concerned, we utilized the antibody, which can recognize a variety of compound structures. We prepared anti-low molecular weight compound antibodies and applied it to target detections.

Research Products

• [Journal Article] Terpendole E and its derivative inhibit STLC- and GSK-1-resistant Eg5 (2014)
  • Author(s): Yuka TARUI, Takumi CHINEN, Yoko NAGUMO, Takayuki MOTOYAMA, Toshiaki HAYASHI, Hiroshi HIROTA, Makoto MUROI, Yasuyuki ISHII, Hisae KONDO, Hiroyuki OSADA, Takeo USUI
  • Journal Title: ChemBioChem Volume: 15 Pages: 934-938
• [Journal Article] The reversible increase in tight junction permeability induced by capsaicin is mediated via cofilin-actin cytoskeletal dynamics and decreased level of occludin (2013)
  • Author(s): Tomoko SHIOBARA, Takeo USUI, Junkyu HAN, Hiroko ISODA, Yoko NAGUMO
  • Journal Title: PLoS ONE Volume: 8 Pages: e79954
  • DOI: 10.1371/journal.pone.0079954
• [Journal Article] Glaziovianin A prevents endosome maturation via inhibiting microtubule dynamics (2013)
  • Author(s): Takumi CHINEN, Sayaka KAZAMI, Yoko NAGUMO, Ichiro HAYAKAWA, Akiyuki IKEDO, Masatoshi TAKAGI, Akihito YOKOSUKA, Naoko IMAMOTO, Yoshihiro MIMAKI, Hideo KIGOSHI, Hiroyuki OSADA, Takeo USUI
  • Journal Title: ACS Chem. Biol Volume: 8 Pages: 884-889
• [Journal Article] Defining the molecular response to trastuzumab, pertuzumab and combination therapy in ovarian cancer (2012)
  • Author(s): Andrew SIMS, Annelien ZWEEMER, Yoko NAGUMO, Dana FARATIAN, Morwenna MUIR, Michael DODDS, Inhwa UM, Charlene KAY, Max HASMANN, David HARRISON, Simon LANGDON
  • Journal Title: British. J. Cancer Volume: 106 Pages: 1779-1789
• [Presentation] Mode of action of anti-HER2 antibodies in ovarian cancer (2013)
  • Author(s): Yoko NAGUMO, Andrew SIMS, Annelien ZWEEMER, Dana FARATIAN, David HARRISON, Simon LANGDON
  • Organizer: 日本薬学会大133年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2013-03-30
• [Presentation] Capsaicinによる可逆的タイトジャンクション開口メカニズムの解析 (2013)
  • Author(s): 塩原智子, 臼井健郎, 韓畯奎, 礒田博子, 南雲陽子
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2013-03-25
• [Presentation] Extacting Various Functions from Food Ingredients : Case Study about Capsaicin (2012)
  • Author(s): Yoko NAGUMO, Junkyu HAN, Hiroko ISODA
  • Organizer: Tunisia-Japan Symposium on Science, Society and Technology (TJASSST)
  • Place of Presentation: Hammamet (Tunisia)
  • Year and Date: 2012-11-17
• [Remarks]
  • URL: http://www.agbi.tsukuba.ac.jp/~usui/study/top.html