2013 Fiscal Year Final Research Report
Structural basis for plant-specific type III polyketide synthase based on the X-ray crystal structure analysis and computational chemistry
| Project/Area Number |
24710246
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Living organism molecular science
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| Research Institution | University of Toyama |
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Principal Investigator |
MORITA Hiroyuki 富山大学, 和漢医薬学総合研究所, 教授 (20416663)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 生合成 / ポリケタイド合成酵素 / 酵素工学 / ポリフェノール / アルカロイド |
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| Research Abstract |
We solved crystal structure of an octaketide synthase (OKS) F66L/N222G mutant enzyme that catalyzes the longest number of malonyl-CoA condensations to produce TW95a. Furthermore, we succeeded in producing an OKS mutant enzyme that increased enzyme activity for the formation of TW95a to approximately 1.5 times on the basis of the crystal structure. In addition, we succeeded in cloning a novel type III PKS that specifically catalyzes the formation of quinolinone.
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