Development of a novel ligand screening system for G protein-coupled receptors and its application

2014 Fiscal Year Final Research Report

• Project/Area Number: 24710247
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Living organism molecular science
• Research Institution: The University of Tokyo
• Principal Investigator: IIZUKA Ryo 東京大学, 薬学研究科(研究院), 助教 (90541954)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: Gタンパク質共役受容体 / 進化分子工学 / 出芽酵母 / マイクロ流体デバイス

Outline of Final Research Achievements

G protein-coupled receptors (GPCRs) are particularly important drug targets. Thus, intense efforts have been devoted to screening novel ligands for GPCRs, which can lead to potential drug candidates. However, for many GPCRs, such efforts have failed to yield desired results. To overcome the problem, I envisioned a directed evolution system to generate agonistic peptides for GPCRs using water-in-oil emulsions. In this study, I established basic techniques for efficient implementation of the system.

Free Research Field

生物物理学