2013 Fiscal Year Final Research Report

Mode of action study of tripropeptin C, which exhibits excellent in vivo efficacy superior to vancomycin

Research Project

Project/Area Number	24710254
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Living organism molecular science
Research Institution	Microbial Chemistry Research Foundation
Principal Investigator	HASHIZUME Hideki 公益財団法人微生物化学研究会, 微生物化学研究所, 主任研究員 (10311276)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	薬剤耐性菌 / 院内感染 / トリプロペプチン / バンコマイシン / MRSA
Research Abstract	Natural lipopeptide antibiotic tripropeptin C (TPPC) exhibits excellent in vivo efficacy in a mice-MRSA septicemia model. MRSA is well-known as multi-drug resistant, major nosocomial pathogen. The purpose of this study was to reveal how TPPC acts against MRSA. We found carboxylic acids in TPPC molecule are essential to exhibit antimicrobial activity. And we revealed the dissociation constant between TPPC and its target molecule UPP. We accomplished to produce isotope-labeled TPPC for the analysis of molecular based interaction by using solid-state NMR.