2013 Fiscal Year Final Research Report
Structure and dynamics measurements of thin filaments containing cardiomyopathy-causing mutant of troponin
| Project/Area Number |
24710255
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Living organism molecular science
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| Research Institution | Japan Atomic Energy Agency |
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Principal Investigator |
MATSUO Tatsuhito 独立行政法人日本原子力研究開発機構, 原子力科学研究部門 量子ビーム応用研究センター, 研究員 (60623907)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | トロポニン / 中性子 / X線 / 心筋症 |
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| Research Abstract |
The effect of the cardiomyopathy-causing mutation in human cardiac troponin on its structure and dynamics was investigated. Troponin complex containing wild-type TnT2 or K247R mutant of TnT2 were reconstituted. Neutron scattering experiments on these solution samples have shown that the mutation causes the troponin molecule to be softer than the wild-type. The structural change of the native thin filament purified from a bovine heart upon calcium binding to TnC was detected by small-angle x-ray scattering (SAXS) experiments. The experiments to measure the structural change of the thin filament caused by the mutation of troponin are under way.
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