2013 Fiscal Year Final Research Report
Functional analysis for PKC which localized on organelle utilizing fatty acid-induced cell death
| Project/Area Number |
24710268
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
TAMURA Yuki 独立行政法人理化学研究所, 袖岡有機合成化学研究室, 客員研究員 (50442984)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | プロテインキナーゼＣ / 脂肪酸 / 細胞死 |
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| Research Abstract |
GLA-induced translocation of several PKC subtypes were observed under fluorescence microscope, and PKCdelta and zeta localized around the nucleus in HeLa cells. Fatty acids accumulated as lipid droplet around the nucleus, which were confirmed by using Nile Red staining and Raman microscope. However, translocated PKCdelta did not seem to co-localize with lipid droplets, suggesting that PKCdelta did not translocate to lipid droplets and might localize at the other organelles such as Golgi apparatus, Endoplasmic reticulum or mitochondria.
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