Monitoring of the ratio of floral morph in endangered plant Primula genus using the heterostylous gene

2015 Fiscal Year Final Research Report

• Project/Area Number: 24710270
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Resource conservation science
• Research Institution: Kobe University
• Principal Investigator: YOSHIDA YASUKO 神戸大学, (連合)農学研究科(研究院), 助教 (50582657)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 異型花柱性 / 絶滅危惧種 / サクラソウ / モニタリング / RNA-seq

Outline of Final Research Achievements

Primula sieboldii, a perennial clonal herb, is distributed in Japan and eastern Asia. Overexploitation and destruction of its habitats and horticultural collection are threatening wild populations with extinction, and the species has become endangered in Japan. Seed production is limited in small populations in which the ratio of the two morphs (long-styled and short-styled) is imbalanced; this ratio is very important for reproductive success. The aim in this study was to develop molecular markers associated with the two morphs in a distylous wild population. These markers are likely to be useful tools for monitoring the ratio of morphs in a population containing the non-flowering ramets or during seasons when the ramets are not flowering. The result of RNA sequencing, specific single-nucleotide polymorphisms were detected in short-styled morph. Using these informations, developing molecular markers are aim for morph identification.

Free Research Field

保全遺伝学