2013 Fiscal Year Final Research Report
Development of fluorescent probes for visualizing low-pH environment and their application in intravital imaging
| Project/Area Number |
24750160
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Osaka University |
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Principal Investigator |
KOWADA Toshiyuki 大阪大学, 免疫学フロンティア研究センター, 特任助教 (40584397)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 破骨細胞 / 蛍光プローブ / イメージング / pH感受性 |
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| Research Abstract |
To perform intravital fluorescence imaging of osteoclasts, which relate to bone diseases such as rheumatoid arthritis and osteoporosis, we developed fluorescent probes that can visualize low-pH region created during bone resorption. A bisphosphonate group and photo-induced electron transfer mechanism were used for the delivery and controlling pH-dependent fluorescence of the probes. In addition, we achieved long-term imaging of osteoclast activity in vivo with improved probe that has electron-withdrawing groups to enhance its photostability. Furthermore, we succeeded to develop the fluorescent probes with red-shifted emission maxima.
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[Journal Article] Dynamic visualization of RANKL-mediated functional control of multinucleate osteoclast function in vivo2013
Author(s)
Junichi Kikuta, Yoh Wada, Toshiyuki Kowada, Ze Wang, Ge-Hong Sun-Wada, Issei Nishiyama, Shin Mizukami, Nobuhiko Maiya, Hisataka Yasuda, Atsushi Kumanogoh, Kazuya Kikuchi, Ronald N. Germain, Masaru Ishii
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Journal Title
J.Clin.Invest.
Volume: 123
Pages: 866–873
DOI
Peer Reviewed
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