2013 Fiscal Year Final Research Report
X-ray crystallography of quasi-racemic glycoprotein synthesized by chemical methods
| Project/Area Number |
24750161
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Osaka University |
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Principal Investigator |
OKAMOTO Ryo 大阪大学, 理学(系)研究科(研究院), 助教 (30596870)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 糖タンパク質 / 結晶構造解析 / N-アセチルグアニジン / ペプチドグアニジド / ペプチドチオエステル / 不凍糖タンパク質 |
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| Research Abstract |
In this study, a new peptide-thioester synthetic method and a regioselective peptide coupling reaction using peptide-guanidide, that was originally found by the author, have been successfully established for the synthesis of homogeneous glycoproteins. Based on these new methodologies, the syntheses of N-glycoprotein: CD59 and O-linked glycoprotein: anti freeze glycoprotein (AFGP) were also achieved. Furthermore, a diastereomeric form of AFGP which has D-amino acids and native GalNAc residues have also successfully synthesized. Quasi-racemic crystallization of AFGP was conducted by using the solution of the 1:1 mixture of the native AFGP and the diastereomeric AFGP.
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