Functional molecular arrangements utilizing protein oligomerization by domain swapping

2014 Fiscal Year Final Research Report

• Project/Area Number: 24750163
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Chemistry related to living body
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: NAGAO Satoshi 奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (30452535)
• Research Collaborator: HIROTA Shun 奈良先端科学技術大学院大学, 物質創成科学研究科, 教授 ; KAMIKUBO Hironari 奈良先端科学技術大学院大学, 物質創成科学研究科, 准教授 ; TOMIOKA Yuya ; MOROI Maki
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: タンパク質超分子 / 機能性分子 / ドメインスワッピング / 多量体

Outline of Final Research Achievements

Supramolecules have been known as the molecules assembled with the regulated intermolecular interactions. In this research, we tried to arrange functional molecules with protein oligomerization by domain swapping and investigated their structure and function.
We succeeded to construct zinc-substituted cytochrome c oligomers from monomers, and showed that the structural and photochemical properties did not change after oligomerization. We also showed that the carbon monoxide binding rate constant of dimeric myoglobin was about twice larger than that of the monomer, although the active site structure was similar between the monomer and dimer. We showed that cytochrome c551 formed dimers by domain swapping and retained the redox potential after dimerization. These results suggest that protein oligomerization by domain swapping can be useful to arrange functional molecules.

Free Research Field

タンパク質科学