• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2013 Fiscal Year Final Research Report

Creation of a Supramolecular CO Receptor for Investigation of Biological Roles of Endogenously-Produced Internal CO

Research Project

  • PDF
Project/Area Number 24750165
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Chemistry related to living body
Research InstitutionDoshisha University

Principal Investigator

KITAGISHI Hiroaki  同志社大学, 理工学部, 助教 (60448090)

Project Period (FY) 2012 – 2013
Keywords生体機能関連化学 / ヘムタンパク質モデル
Research Abstract

Carbon monoxide (CO) is continuously produced in the eukaryotic organisms during degradation of hemin by heme oxygenase (HO). The biological roles of CO in the living organisms have not been fully understood because of lacking of knockdown method for endogenously produced CO. We have shown that a supramolecular complex, hemoCD, strongly binds CO higher than that of hemoglobin. In the present study, hemoCD was used for removal of CO in the living organisms and genetic response upon removal of CO was investigated. Furthermore, for monitoring the depletion effect of CO in the human cells, the hemoCD scaffold was conjugated with an octaarginine peptide that has a strong ability to bring molecules into the cell interior. This conjugate, R8-hemoCD, was smoothly taken by the HeLa cells without significant toxicities.

  • Research Products

    (12 results)

All 2014 2013 2012 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (7 results) Remarks (1 results)

  • [Journal Article] Intracellular Delivery of Anionic meso-Tetraarylporphyrin/Per-O-methylated β-Cyclodextrin Supramolecular Complexes by an Oligoarginine Carrier Peptide2014

    • Author(s)
      H. Kitagishi, K. Minami, K. Kano
    • Journal Title

      Chem. Lett.

      Volume: (in press)

    • DOI

      DOI:10.1246/cl.140298

    • Peer Reviewed
  • [Journal Article] Cellular Uptake of Octaarginine-conjugated Tetraarylporphyrin Included by Per-O-methylated β-Cyclodextrin2013

    • Author(s)
      H. Kitagishi, S. Hatada, T. Itakura, Y. Maki, Y. Maeda, K. Kano
    • Journal Title

      Org. Bio. Chem.

      Volume: 11(19) Pages: 3203-3211

    • Peer Reviewed
  • [Journal Article] PEGylation of an Artificial O_2 and CO Receptor : Synthesis, Characterisation and Pharmacokinetic Study2012

    • Author(s)
      T. Ueda, H. Kitagishi, K. Kano
    • Journal Title

      Org. Biomol. Chem.

      Volume: 10(22) Pages: 4337-4347

    • Peer Reviewed
  • [Journal Article] Functionalization of a Protein Surface with Per-O-methylated β-Cyclodextrin2012

    • Author(s)
      H. Kitagishi, K. Kashiwa, K. Kano
    • Journal Title

      Biopolymers

      Volume: 97(1) Pages: 11-20

    • Peer Reviewed
  • [Presentation] 超分子COレセプターの細胞内導入法の検討2014

    • Author(s)
      南景子, 北岸宏亮, 加納航治
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋
    • Year and Date
      2014-03-27
  • [Presentation] 超分子COレセプターによる生体内COの除去効果2014

    • Author(s)
      勇村亞希, 北岸宏亮, 根木 滋, 杉浦幸雄, 加納航治
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋
    • Year and Date
      2014-03-27
  • [Presentation] 鉄(II)ポルフィリン/シクロデキストリン超分子錯体による内因性一酸化炭素の除去とそれによる細胞および動物体内における遺伝子発現変化2013

    • Author(s)
      北岸宏亮, 勇村亞希, 加納航治
    • Organizer
      錯体化学会第63回討論会
    • Place of Presentation
      沖縄
    • Year and Date
      2013-11-02
  • [Presentation] メチル化シクロデキストリン二量体で包接された鉄(III)ポルフィリンによる水中でのアニオン認識2013

    • Author(s)
      北岸宏亮, 齊藤友紀恵, 菅谷陽輔, 加納航治
    • Organizer
      第30回シクロデキストリンシンポジウム
    • Place of Presentation
      熊本
    • Year and Date
      2013-09-13
  • [Presentation] Genetic response upon removal of endogenous CO from the blood of mice by administration of a synthetic CO receptor2013

    • Author(s)
      H. Kitagishi, S. Negi, Y. Mizukawa, Y. Amagase, A. Yumura, T. Urushidani, Y. Sugiura, K. Kano
    • Organizer
      16^<th> International Conference on Bio-inorganic Chemistry (ICBIC16)
    • Place of Presentation
      Grenoble
    • Year and Date
      2013-07-23
  • [Presentation] Capture of Endogenous Carbon Monoxide by an Iron(II)porphyrin-Cyclodextrin Supramolecular Complex2012

    • Author(s)
      H. Kitagishi, S. Negi, S. Hatada, Y. Maki, Y. Maeda, Y. Sugiura, K. Kano
    • Organizer
      7^<th> International Conference on Porphyrins and Phthalocyanines
    • Place of Presentation
      Jeju, Korea
    • Year and Date
      20120701-06
  • [Presentation] オクタアルギニン連結ポルフィリンの細胞内導入におけるシクロデキストリン包接効果2012

    • Author(s)
      北岸宏亮, 畠田智史, 加納航治
    • Organizer
      第29回シクロデキストリンシンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2012-09-06
  • [Remarks]

    • URL

      http://www1.doshisha.ac.jp/~kkano/

URL: 

Published: 2015-03-12  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi