2013 Fiscal Year Final Research Report
Relationship between intracellular mechanical field and mechanosensors in stretched cell
| Project/Area Number |
24760078
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Materials/Mechanics of materials
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| Research Institution | The University of Tokushima |
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Principal Investigator |
SATO Katsuya 徳島大学, ソシオテクノサイエンス研究部, 講師 (10403651)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 細胞力覚 / 骨芽細胞 / 伸展刺激 / 力学的刺激 / バイオメカニクス / MEMS |
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| Research Abstract |
In this study, we developed a novel cell stretching micro device to observe cellular response to stretching stimuli with high spatial and temporal resolution.
First, we have modified our stretching device to reduce the focus drift during the stretching. And we have introduced the fluorescent ratiometric measurement to reduce influence of change in fluorescent intensity arose from the focus drift. Finally, we succeed to conduct observation of cellular response to stretching stimuli with high spatial and temporal resolution.
Second, we developed an experimental system to evaluate the intracellular strain field in stretched cell. We combined our cell stretching micro device and the image correlation method to measure the strain in stretched cell. We succeed to evaluated the strain field in the cell during stretch.
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