2013 Fiscal Year Final Research Report
Temperature-modulated selective cell adhesion and detachment using intelligent bio-interface and application of them to cell separation
| Project/Area Number |
24760580
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural/Functional materials
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
NAGASE Kenichi 東京女子医科大学, 医学部, 講師 (10439838)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | バイオマテリアル / 細胞分離 / インテリジェント材料 / 温度応答性 / ポリマーブラシ |
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| Research Abstract |
For preparing thermally modulated bio-interface that separates cells without the modification of cell surfaces for regenerative medicine and tissue engineering, thermo-responsive hydrophobic copolymer brushes were formed on glass substrate. Prepared copolymer brush surfaces were characterized by observing the adhesion at 37 C and detachment at 20 C or 10 C of four types of human cells. Endothelial cell and fibroblast exhibited their effective detachment temperature at 20 C and 10 C, respectively. Using cells' intrinsic temperature sensitivity for detachment from the copolymer brush, a mixture of endothelial cell and fibroblast was separated.
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