Identification and characterization of a novel functional acylhomoserine lactone-degrading enzyme for preventing bacterial infection

2014 Fiscal Year Final Research Report

• Project/Area Number: 24760643
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Utsunomiya University
• Principal Investigator: MOROHOSHI Tomohiro 宇都宮大学, 工学(系)研究科(研究院), 准教授 (90361360)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: クオラムセンシング / アシル化ホモセリンラクトン / Thermaerobacter / ラクトナーゼ / 分解

Outline of Final Research Achievements

Thermaerobacter marianensis is an extremely thermophilic bacterium. N-Acylhomoserine lactone (AHL) is a quorum-sensing signal molecule used by many gram-negative bacteria. We found AHL-degrading gene homologue (designated aiiT) in the genome sequences of T. marianensis JCM 10246. AiiT has 59.7%, 21.2, and 11.2% identities with AhlS from Solibacillus silvestris, AiiA from Bacillus cereus, and AidC from Chryseobacterium sp., respectively. Purified AiiT, as a maltose-binding fusion protein, showed high AHL-degrading activity against all tested AHLs at the temperatures ranging from 40 to 80°C. AiiT displayed its maximal activity at high temperature range, 60 to 80°C, and showed higher thermostability than the other AHL lactonases.

Free Research Field

分子生物学