2013 Fiscal Year Final Research Report
Development of a novel molecular combing method for high throughput analysis of giant DNA molecule
| Project/Area Number |
24760648
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Toyohashi University of Technology |
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Principal Investigator |
KURITA Hirofumi 豊橋技術科学大学, 工学(系)研究科(研究院), 助教 (70512177)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | DNA伸張固定 / 分子コーミング |
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| Research Abstract |
Molecular combing is a powerful method for aligning giant DNA molecules and used in a wide range of applications from molecular biology to nanoelectronics. The purpose of this study is development of a novel molecular combing method for high throughput analysis of giant DNA molecules. The conventional molecular combing is based on the force applied at the air/water interface. This study proposes to use the oil/water interface to stretch and immobilize giant DNA molecules. As a result, by combining the oil/water interface and a microfluidic channel, the proposing molecular combing was achieved. This combing method based on the novel concept is expected to be a new tool in high throughput analysis of genomic DNA..
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