2014 Fiscal Year Final Research Report
Initiation of nuclear divisions and restoration of diploidy in parthenogenetic embryos of Drosophila ananassae
| Project/Area Number |
24770006
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Kyorin University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 遺伝学 / 二倍体 / 中心体 / 受精 / 単為発生 / ショウジョウバエ / ゲノム / 細胞周期 |
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| Outline of Final Research Achievements |
Parthenogenesis is the development of an unfertilized egg into a new individual. We here examined the mechanisms by which the following problems are solved in parthenogenetic strains of Drosophila ananassae: Mitotic division of the female pronucleus and restoration of diploidy. We found that, in unfertilized eggs of both sexual and parthenogenetic strains, an acentrosomal spindle is assembled around the chromosomes of the female pronucleus, after completion of S phase. It is striking that eggs that were laid by parthenogenetic females assembled centrosome-like structures de novo, which were scattered in the anterior part of the egg cytoplasm. Our data suggest a role of the centrosome-like structures in stimulating the nuclear division of the female pronucleus. We also found that the first diploid nucleus is formed by fusion of two haploid chromosome sets at telophase of the second nuclear cycle of parthenogenetic embryos, resembling the gonomeric division in sexual development.
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| Free Research Field |
細胞遺伝学
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