Transcriptome analysis and laser manipulation for plant axis formation

2014 Fiscal Year Final Research Report

• Project/Area Number: 24770045
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nagoya University (2013-2014); Nara Institute of Science and Technology (2012)
• Principal Investigator: MINAKO Ueda 名古屋大学, 理学研究科(WPI), 講師 (20598726)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 植物発生 / 転写因子 / 体軸形成

Outline of Final Research Achievements

We screened the downstream candidates of a specific transcription factor, which regulates the polarization of the zygote and the axis formation of the early embryo in a model plant, Arabidopsis. Based on the transcriptome analysis using next generation sequence, we identified 27 candidates, including various factors, such as the putative regulator for the organelle structure or for the transport of a major plant hormone. We analyzed the mutants of these genes, and found that several lines are defective in embryo morphology and are embryo lethal in single or multiple mutants.

Free Research Field

植物分子遺伝学