Regulation of chloroplast differentiation during sink/source conversion

2013 Fiscal Year Final Research Report

• Project/Area Number: 24770055
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The University of Tokyo
• Principal Investigator: KOBAYASHI Koichi 東京大学, 総合文化研究科, 助教 (40587945)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 葉緑体 / 光合成 / サイトカイニン / 色素体 / シロイヌナズナ / 緑化

Research Abstract

To elucidate coordination mechanisms of chloroplast differentiation with organ development in plants, greening mechanisms of Arabidopsis roots after shoot removal were examined. The results revealed that cytokinin signaling upregulates photosynthesis-associated genes and improves photosynthetic electron transport efficiency in roots after shoot removal. The cytokinin signaling involves a wounding-responsive factor in upstream and chloroplast-related transcription factors in downstream. Not only Arabidopsis thaliana, other angiosperms such as Dandelion (Traxacum officinale) also improved photosynthetic efficiency in the root after shoot removal. Further analyses showed that photosynthesis in the root is crucial for regeneration of the shoot in Dandelion.

Research Products

• [Journal Article] Photosynthesis of root chloroplasts developed in Arabidopsis lines overexpressing GOLDEN2-LIKE transcription factors (2013)
  • Author(s): Kobayashi K, Sasaki D, Noguchi K, Fujinuma D, Komatsu H, Kobayashi M, Sato M, Toyooka K, Sugimoto K, Niyogi KK, Wada H, Masuda T
  • Journal Title: Plant Cell Physiol Volume: 54 Pages: 1365-1377
  • DOI: 10.1093/pcp/pct086
  • Peer Reviewed
• [Journal Article] Role of galactolipid biosynthesis in coordinated development of photosynthetic complexes and thylakoid membranes during chloroplast biogenesis in Arabidopsis (2013)
  • Author(s): Kobayashi K, Narise T, Sonoike K, Hashimoto H, Sato N, Kondo M, Nishimura M, Toyooka K, Sato M, Sugimoto K, Wada H, Masuda T, Ohta H
  • Journal Title: Plant J Volume: 73 Pages: 250-261
  • DOI: 10.1111/tpj.12028
  • Peer Reviewed
• [Journal Article] Role of the G-box element in regulation of chlorophyll biosynthesis in Arabidopsis roots (2012)
  • Author(s): Kobayashi K, Obayashi T, Masuda T
  • Journal Title: Plant Signal. Behav. Volume: 7 Pages: 922-926
  • DOI: 10.4161/psb.20760
  • Peer Reviewed
• [Journal Article] 高等植物のクロロフィル合成系の制御 (2012)
  • Author(s): 小林康一
  • Journal Title: 光合成研究 Volume: 22 Pages: 125-138
  • URL: http://photosyn.jp/journal/sections/kaiho64-9.pdf
  • Peer Reviewed
• [Presentation] シロイヌナズナにおける誘導的人工マイクロRNAを用いた主要葉緑体膜脂質MGDGの機能解析 (2014)
  • Author(s): 藤井祥、小林康一、Krishna K. Niyogi、中村友輝、和田元
  • Organizer: 第55回植物生理学会年会
  • Place of Presentation: 富山大学
  • Year and Date: 20140318-20
• [Presentation] Photosynthetic characteristics of phosphatidylglycerol-deficient Arabidopsis mutant (2014)
  • Author(s): Kobayashi K, Endo K, Hori H, Fujii S, Niyogi KK, Wada H
  • Organizer: 第55回植物生理学会年会
  • Place of Presentation: 富山大学
  • Year and Date: 20140318-20
• [Presentation] Inducible knockdown of MGD1 in Arabidopsis (2013)
  • Author(s): Fujii S, Kobayashi K, Niyogi KK, Nakamura Y, Wada H
  • Organizer: 5^<th> Asian Symposium on Plant Lipids
  • Place of Presentation: Gwangju, Korea
  • Year and Date: 20131129-1201
• [Presentation] Role of phosphatidylglycerol in Arabidopsis chloroplasts (2013)
  • Author(s): Kobayashi K, Endo K, Hori H, Fujii S, Niyogi KK, Wada H
  • Organizer: 5^<th> Asian Symposium on Plant Lipids
  • Place of Presentation: Gwangju, Korea
  • Year and Date: 20131129-1201
• [Presentation] 傷害に応答した根の緑化に関わるサイトカイニンシグナル経路 (2013)
  • Author(s): 佐々木大地,岩瀬哲,杉本慶子,増田建,和田元,小林康一
  • Organizer: 第54回植物生理学会年会
  • Place of Presentation: 岡山大学
  • Year and Date: 20130321-23
• [Presentation] Photosynthesis in roots of Arabidopsis overexpressing GLK (2013)
  • Author(s): Kobayashi K, Sasaki D, Noguchi K, Fujinuma D, Kobayashi M, Sato M, Toyooka K, Sugimoto K, Wada H, Masuda T
  • Organizer: 第54回植物生理学会年会
  • Place of Presentation: 岡山大学
  • Year and Date: 20130321-23
• [Remarks]
  • URL: http://hajimewada.c.u-tokyo.ac.jp