2013 Fiscal Year Final Research Report

Structural basis of the filament formation of ESCRT-III proteins

Research Project

Project/Area Number	24770094
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Structural biochemistry
Research Institution	University of Toyama
Principal Investigator	OBITA TAKAYUKI 富山大学, 大学院医学薬学研究部(薬学), 准教授 (30578696)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	構造生物学 / 細胞分裂 / ESCRT / 古細菌
Research Abstract	We have cloned several ESCRT-III genes from Yeast or Archaea and made plasmids for protein expression. Although we have purified wild-type and mutants and tested crystallization, we have not got the good diffracting crystals, yet. We have determined the crystal structures of Archaeal CdvA, which interact with ESCRT-III proteins, and revealed that CdvA forms the filament structure. We have also shown the filament structure of CdvA using electron microscopy. Taken together, we have succeeded to show the structural basis of the filament structure of CdvA.