2013 Fiscal Year Final Research Report
The transport mechanism of the peptide transporter by the X-ray structual anaysis.
| Project/Area Number |
24770095
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Tohoku University (2013)
Kyoto University (2012) |
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Principal Investigator |
OGASAWARA Satoshi 東北大学, 医学(系)研究科(研究院), 助教 (30546685)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | ペプチドトランスポーター / モノクローナル抗体 / 構造解析 / 膜蛋白質 |
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| Research Abstract |
We were trying to co-crystallize the complex of the PepTSo and antibody. Furthermore we tried to generate antibodies against PEPT homologues, one homologue was from Streptococcus thermophilus (PepTSt) and two were from Arabidopsis thaliana (PTR1 and PTR2). As we generated antibodies against PepTSo, we were successful to produce some antibodies against three homologues. These antibodies recognized conformational epitope of the PEPT homologues. On initial screening by vapor diffusion method, the complex of the PepTSt and this antidody conformed crystals. The crystals were diffracted at about 3.5 angstrom. We are now proceeding the functional and structural analyses.
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