2014 Fiscal Year Final Research Report
X-ray crystallography of flavonoid metabolon
| Project/Area Number |
24770096
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
MIZOHATA Eiichi 大阪大学, 工学(系)研究科(研究院), 助教 (90571183)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | X線結晶構造解析 |
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| Outline of Final Research Achievements |
Grapevine glycosyltransferase 5 (VvGT5) is a UDP-glucuronic acid:flavonol-3-O-glucuronosyltransferase, catalyzing the 3-O-specific glucuronosylation of flavonols using UDP-glucuronic acid as a sugar donor to produce flavonol 3-O-glucosides, which are important bioactive phytochemicals. In this study, I determined crystal structures of VvGT5 in three kinds of structural states, and revealed structural basis of the sugar donor selectivity of the enzyme during catalysis.
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| Free Research Field |
構造生物学
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