2014 Fiscal Year Final Research Report
X-ray crystallography of Chlorella virus the molecular weight is over billion
| Project/Area Number |
24770097
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 構造生物学 |
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| Outline of Final Research Achievements |
Chlorella virus is a large icosahedral virus of molecular weight over billion with the diameter of about 2000Å. It is reported that the virus has typical structures on the surface, the bi-layer lipid on the inside of the capsid and the genome encodes over 400 proteins. This study aimed to determine the atomic structure of Chlorella virus using by the techniques of X-ray crystallography. The high purity Chlorella virus was prepared on a large scale for the crystal structure analysis. The screening of the crystallization condition was carried out and small crystals were obtained. The fiber structures on the outer layer was clearly observed using the phase contrast cryo-electron microscopy.
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| Free Research Field |
構造生物学
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