2013 Fiscal Year Final Research Report

Analysis of the role of SH3YL1 in the morphology of plasma membrane and endocytosis machinery

Research Project

Project/Area Number	24770098
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Structural biochemistry
Research Institution	Osaka University
Principal Investigator	HASEGAWA Junya 大阪大学, 生命機能研究科, 助教 (00533788)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	イノシトールリン脂質 / エンドサイトーシス
Research Abstract	The ubiquitinated signaling receptors such as the epidermal growth factor (EGF) receptor (EGFR) are delivered to early endosomes, and then sorted to lysosomes for degradation via multivesicular bodies (MVBs). The mechanism underlying the formation and scission of intraluminal vesicles to generate MVBs has remained elusive. In this study, we find that the localization of SH3YL1, which has been found by our group previously, shows the punctate pattern and the puncta is localized close to early endosomes. Deficiency of SH3YL1 prevents EGF trafficking from early to late endosomes, and inhibits the degradation of EGFR. Moreover, we show that loss of SH3YL1 results suppression of EGFR sorting into MVBs, indicating that SH3YL1 is required for the MVB biogenesis. Taken together, our observations reveal that an indispensable role of SH3YL1 in sorting of EGFR and MVB biogenesis.