2014 Fiscal Year Final Research Report
Dynamic behavior of human VRK1 kinase in living cells
| Project/Area Number |
24770108
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Tohoku University (2014)
Institute of Physical and Chemical Research (2012-2013) |
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Principal Investigator |
KOSHIBA Seizo 東北大学, 東北メディカル・メガバンク機構, 准教授 (70332301)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 酵素 / キナーゼ / NMR / In-cell |
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| Outline of Final Research Achievements |
I developed a new method to analyze dynamic behavior of proteins in living cells using In-Cell NMR and fluorine-labeling methods. High-resolution 19F-NMR spectra of VRK1 kinase protein were obtained. This method is useful for understanding the dynamic regulation of proteins under physiological conditions.
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| Free Research Field |
NMR法を用いた生体高分子の構造機能解析
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