2013 Fiscal Year Final Research Report
Super-resolution, multicolor imaging of chromatin structures in the interphase nucleus
Project/Area Number |
24770113
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Structural biochemistry
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Research Institution | National Institute of Information and Communications Technology |
Principal Investigator |
MATSUDA Atsushi 独立行政法人情報通信研究機構, 未来ICT研究所バイオICT研究室, 研究員 (20585723)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Keywords | クロマチン / 高分解能顕微鏡観察 |
Research Abstract |
Fission yeast has been a classical model organism for gene silencing. However, little is known about its chromatin structure due to the small size of the genome. Here we used super-resolution fluorescence microscopy to observe condensation levels of interphase chromatin in fission yeast. Unexpectedly silent chromatin is less condensed than the rest of euchromatin in fission yeast. Furthermore, the subtelomeric silent regions are flanked by highly condensed, but non-silencing chromatin body, which we named as knob. The regions of knob span for about 50 kb devoid of methylated histones. Knob condensation was independent from HP1 homolog swi6. The unusual chromatin structure indicates that gene silencing in fission yeast is not a direct consequence of chromatin condensation.
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Research Products
(19 results)
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[Journal Article] Autophagosomes form at ER-mitochondria contact sites2013
Author(s)
Maho Hamasaki, Nobumichi Furuta, Atsushi Matsuda, Akiko Nezu, Akitsugu Yamamoto, Naonobu Fujita, Hiroko Oomori, Takeshi Noda, Tokuko Haraguchi, Yasushi Hiraoka, Tamotsu Yoshimori and Atsuo Amano
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Journal Title
Nature
Volume: 495
Pages: 389-393
DOI
Peer Reviewed
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[Presentation] デノイジング2013
Author(s)
松田厚志
Organizer
第21回細胞生物学ワークショップ
Place of Presentation
未来ICT研究所、兵庫県神戸市
Year and Date
2013-08-07
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