2014 Fiscal Year Final Research Report
Elucidation of regulation/control mechanisms in glycan-mediated functions of cell-surface biomolecules.
Project/Area Number |
24770138
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Functional biochemistry
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Research Institution | National Institute of Advanced Industrial Science and Technology |
Principal Investigator |
TOGAYACHI Akira 独立行政法人産業技術総合研究所, 糖鎖創薬技術研究センター, 主任研究員 (60392635)
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Co-Investigator(Renkei-kenkyūsha) |
KAJI Hiroyuki 産業技術総合研究所, 糖鎖創薬技術研究センター・標的糖鎖探索チーム, 研究チーム長 (80214302)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Keywords | 糖鎖 / ポリラクトサミン / 糖転移酵素 / β1,3-N-アセチルグルコサミン転移酵素 / 糖タンパク質 / グライコプロテオミクス解析 / 免疫系 |
Outline of Final Research Achievements |
Glycosyltransferase gene-deficient mice show various abnormalities in biological functions. To analyze the mechanism, it is important to identify the glycoproteins targeted by the glycosyltransferases (glycan carrier molecules). In this study, we focused on polylactosamine glycans and identified the carrier molecules of polylactosaminoglycans using glycoproteomics technology. Glycopeptides were captured with the lectins specifically bind to polylactosamine glycans. Comparative glycoproteome analysis of the captured glycopeptides identified the target glycoprotein candidates. Bioinformatics analysis of the obtained carrier molecules found common characteristics and tendency in the molecular nature.
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Free Research Field |
糖鎖生物学・細胞生物学・生化学
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