2013 Fiscal Year Final Research Report
Reversible photo-regulation of the motor functions of kinesin by a novel photochromic ATP analog
| Project/Area Number |
24770140
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Hokkaido University |
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Principal Investigator |
KAMEI Takashi 北海道大学, 電子科学研究所, 助教 (90450650)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | モータータンパク質 / ATPアナログ / フォトクロミック分子 |
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| Research Abstract |
We obtained the following two main results in this research.
(1) We synthesized novel adenosine triphosphate (ATP) analogs, ATP-Azos, which were chimeric molecules of ATP and azobenzene compounds exhibiting a reversible and large conformational change by photo-irradiations. In aqueous solutions, photo-responsive properties of the ATP-Azos were revealed with spectroscopic methods.
(2) In the presence of an ATP-Azo, we assessed the motile properties of kinesin, a bio-molecular motor, which derives the mechanical energy from ATP hydrolysis, with or without photo-irradiations. Consequently, we realized reversible photo-modulation of motility of kinesin by irradiations.
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