2013 Fiscal Year Final Research Report
Identification of torque generation part in flagellar motor by using fluorescence polarization
| Project/Area Number |
24770144
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Tohoku University |
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Principal Investigator |
FUKUOKA Hajime 東北大学, 多元物質科学研究所, 助教 (50447190)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | バイオイメージング / 分子モーター / べん毛モーター |
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| Research Abstract |
The microscopic system to detect the polarization of fluorescent molecules was develoved. By using this microscopic system, the polarization of fluorescence from a single GFP molecule was detected, and the direction of a GFP molecule on the surface of coverslip was estimated from the fluorescent intensities of GFP in P-polarization and S-polarization. The microscopic system to simultaneously observe the fluorescence from GFP-fusion proteins and the rotation of flagellar motor was also constructed. By using this microscopic system, it was demonstrated that the rotational switch of a motor was directly regulated by the binding and dissociation of phosphorylated CheY by simultaneously visualizing CheY tagged with GFP and the rotational switching of a motor in live cells. The results of this study was published from Sci. Signal. 2014. 7: ra32.
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