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2013 Fiscal Year Final Research Report

A study of the compartment size dependency of the protein synthesis using a cell-free translation system

Research Project

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Project/Area Number 24770153
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionOsaka University

Principal Investigator

OKANO Taiji  大阪大学, 情報科学研究科, 特別科学研究員 (60622082)

Project Period (FY) 2012-04-01 – 2014-03-31
Keywords無細胞翻訳系 / タンパク質合成 / 一分子計測 / マイクロチャンバー / ドライエッチング
Research Abstract

We investigated the compartment size dependency of the protein synthesis reaction using quartz-glass microchambers. By introducing the glass chamber, the concentration of the synthesized protein was significantly improved compared to that in the poly(dimethylsiloxane) (PDMS) microchamber. The concentration was below the detection limit in the PDMS chambers, whereas the glass chambers yielded 41% of the bulk reaction. When the cell-free protein synthesis reaction with different reaction orders was conducted in glass chambers, the reaction dynamics was largely affected by the chamber size although the synthesis reaction was initiated from a constant copy number of DNA. We were able to observe the properties specific to the high order reaction and found the presence of an optimum compartment volume for a high-order reaction using real biological molecules.

  • Research Products

    (8 results)

All 2014 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (6 results)

  • [Journal Article] Cell-free Protein Synthesis in a Microchamber Revealed the Presence of an Optimum Compartment Volume for High-order Reactions2013

    • Author(s)
      T. Okano, T. Matsuura, H. Suzuki, and T. Yomo
    • Journal Title

      ACS Synthetic Biology

    • DOI

      10.1021/sb400087e

    • Peer Reviewed
  • [Journal Article] Cell-free protein synthesis from a single copy of DNA in a glass microchamber2012

    • Author(s)
      T. Okano, T. Matsuura, Y. Kazuta, H. Suzuki, and T. Yomo
    • Journal Title

      Lab on a Chip

      Volume: 12 Pages: 2704–2711

    • Peer Reviewed
  • [Presentation] ガラスマイクロチャンバーを用いたタンパク質合成の反応場体積依存性2014

    • Author(s)
      岡野太治,松浦友亮,鈴木宏明,四方哲也
    • Organizer
      日本機械学会第26回バイオエンジニアリング講演会
    • Place of Presentation
      東北大学片平キャンパスさくらホール
    • Year and Date
      2014-01-11
  • [Presentation] タンパク質合成反応への反応場サイズの寄与2013

    • Author(s)
      岡野太治,松浦友亮,鈴木宏明,四方哲也
    • Organizer
      「細胞を創る」研究会6.0
    • Place of Presentation
      鶴岡市先端研究産業支援センター
    • Year and Date
      20131114-15
  • [Presentation] マイクロウェルを用いたDNA 1分子からのタンパク質合成反応計測2013

    • Author(s)
      岡野太治,松浦友亮,数田恭章,鈴木宏明,四方哲也
    • Organizer
      日本機械学会2013年度年次大会
    • Place of Presentation
      岡山大学津島キャンパス
    • Year and Date
      20130909-11
  • [Presentation] マイクロウェルを用いたDNA 1分子からのタンパク質合成反応計測2013

    • Author(s)
      岡野太治,松浦友亮,数田恭章,鈴木宏明,四方哲也
    • Organizer
      化学とマイクロ・ナノシステム学会第27回研究会
    • Place of Presentation
      東北大学片平キャンパスさくらホール
    • Year and Date
      20130523-24
  • [Presentation] 一分子DNAからのタンパク質合成のリアルタイム計測2012

    • Author(s)
      岡野太治,松浦友亮,数田恭章,鈴木宏明,四方哲也
    • Organizer
      「細胞を創る」研究会5.0
    • Place of Presentation
      東京工業大学すずかけ台キャンパスすずかけホール
    • Year and Date
      20121121-22
  • [Presentation] ガラスマイクロチャンバーを用いた一分子DNAからのタンパク質合成2012

    • Author(s)
      岡野太治,松浦友亮,数田恭章,鈴木宏明,四方哲也
    • Organizer
      平成24年度電気学会センサ・マイクロマシン部門総合研究会
    • Place of Presentation
      京都大学百周年時計台記念館
    • Year and Date
      20120611-12

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Published: 2015-06-25  

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