2013 Fiscal Year Final Research Report
Functional analysis of non-coding RNA in regulation of chromosome segregation
| Project/Area Number |
24770168
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Kumamoto University |
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Principal Investigator |
IDEUE Takashi 熊本大学, 自然科学研究科, 助教 (20420250)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | RNA / 染色体分離 / ヘテロクロマチン |
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| Research Abstract |
Satellite I RNA is noncoding RNA transcribed from human centromeric repetitive sequence. Depletion of this RNA caused the abnormal nuclear morphology due to defects in chromosome segregation. We founded that Aurora kinase B, a key factor of chromosome segregation binds to this RNA. Aurora B was regulated kinase activity and localization through binding to this RNA. To identify interaction factors to satellite I RNA, we performed pulled down of Satellite I RNA. Several interactors were identified from pulled down products by LC/MS analysis. Depletion of RBMX, one of interaction protein, showed phenotype of defect in chromosome segregation.
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