2014 Fiscal Year Final Research Report
Actin and myosin-II interaction of the contractile ring in fission yeast
| Project/Area Number |
24770177
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 細胞質分裂 / アクチン細胞骨格 / II型ミオシン / 収縮環 / 分裂酵母 / IQGAP / アクチン調節タンパク質 / 分子モーター |
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| Outline of Final Research Achievements |
Cytokinesis is essential for proliferation of any type of cell. Especially in animal cells and fungi, the contractile ring --a circular structure that is composed of actin filaments and myosin-II like muscle sarcomere-- plays an important role in the spatiotemporally regulated cytofission. In this study, we aimed to elucidate the molecular basis of actin-myosin-II interaction in the contractile ring. We found that an actin-binding protein that localized to the ring and is vital for its assembly, Rng2, regulated ATPase and motor activities of myosin-II and that myosin-II was involved in maintenance of the ring at later anaphase, not only in its assembly and constriction. These findings may greatly contribute to a comprehensive understanding of the whole mechanism of cytokinesis at molecular level and to medical applications, for example development of a technique for regulating growth of some type of cell such as cancer cells.
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| Free Research Field |
細胞生物学
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