2014 Fiscal Year Final Research Report
Comparative phospho-motif analysis reveals the evolutionary expansion of phosphorylation signaling networks
| Project/Area Number |
24770190
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | リン酸化 / シグナル伝達 / 比較進化解析 / モチーフ解析 |
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| Outline of Final Research Achievements |
Protein phosphorylation is a post-translational modification that is essential for a wide range of eukaryotic physiological processes. However the physiological roles of most remain unknown. In this study, we developed a method to extract phosphosites with important roles in cellular functions.
We determined 178 phosphomotifs based on the analysis of 34,366 phosphosites. Comparative genomic analyses were performed using genomes from nine species from yeast to humans. Consequently, we identified 16 phosphomotifs, in which the level of conservation increased among species. The highly conserved phosphomotifs in humans and the worm were kinase regulatory sites. The motifs that appeared in the fly were novel phosphomotifs, including zinc finger motifs. The motifs that appeared in fish allowed the detection of the expansion of phosphorylation signaling related to alternative splicing.
Our method can be helpful for extracting novel phosphomotifs with physiological functions.
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| Free Research Field |
細胞生物学
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