2013 Fiscal Year Final Research Report
Analyses of nuclear envelope dynamics using a novel model fission yeast
| Project/Area Number |
24770192
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
AOKI KEITA 国立遺伝学研究所, 系統生物研究センター, 助教 (20620884)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 核膜動態 / 核膜崩壊 / 分裂期制御 / 細胞周期 / S. japonicus |
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| Research Abstract |
For the inheritance of genetical information, a nuclear membrane, surrounding DNA, has to be inherited to daughter cells correctly. Higher eukariotic cells like human have 'open' mitosis system accompanying breakdown and regeneration of nuclear membrane. On the other hand, lower eukariotic cells like yeasts have 'closed' mitosis system accompanying shrinkage of nuclear membrane. The significance and mechanism of the difference has not been understood so far.
For our study, a fission yeast S. japonicus, which has 'semi-open' mitosis, was used. Searching genes relating to inheritance of nuclear membrane by genetical screening, it was found that APC/Cyclosome and fatty acid synthase function for the inheritance of nuclear membrane. Because APC/Cyclosome is a key factor in the protein degradation pathway, and fatty acids are important for constructing membrane, we proposed a model that regulation of level of nuclear membrane via protein degradation is necessary for the inheritance of DNA.
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