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2014 Fiscal Year Final Research Report

Novel shRNA library screening to identify key factors that are important in pluripotent stem cells

Research Project

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Project/Area Number 24770206
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Developmental biology
Research InstitutionKyoto University

Principal Investigator

HOTTA Akitsu  京都大学, iPS細胞研究所, 特定拠点助教 (50578002)

Project Period (FY) 2012-04-01 – 2015-03-31
KeywordsshRNAライブラリー / トランスポゾンベクター / 発現抑制制御
Outline of Final Research Achievements

To reveal the molecular mechanisms behind the pluripotency of ES cells and iPS cells, we constructed a piggyBac DNA transposon vector to deliver a genome-wide shRNA library into ES/iPS cells. The generated shRNA library was validated by deep sequencing, and several ten-thousand shRNAs were successfully cloned into our piggyBac vector. Our piggyBac shRNA library is a versatile tool to dissect the molecular mechanisms in pluripotent stem cells and other cell types, without the use of viral vectors.

Free Research Field

幹細胞遺伝子工学

URL: 

Published: 2016-06-03  

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