2013 Fiscal Year Final Research Report
Elucidation of essential growth factors for mouse spermatogenesis using organ culture method.
| Project/Area Number |
24770216
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
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| Research Institution | Yokohama City University |
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Principal Investigator |
SATO Takuya 横浜市立大学, 生命医科学研究科, 特任助教 (70599505)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 精子形成 / 器官培養 / 精子幹細胞 / 男性不妊 / Stem cell factor |
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| Research Abstract |
In the present study, using a testis tissue culture method, we challenged to treat a spermatogenic defect mutant without any genetic manipulations. The Sl/Sld mouse has only a few primitive spermatogonia as germ cells in the testis, lacking any sign of spermatogenesis, due to mutations of the c-Kit ligand gene (Kitl) which cause the loss of membrane-bound-type KITL from the surface of Sertoli cells. We succeeded in inducing the complete spermatogenesis of Sl/Sld mutant mice by culturing their testis tissues in culture media containing KITL and colony stimulating factor-1 (CSF-1).To elucidate the mechanism of CSF-1's effect on spermatogenesis, we performed transplantation experiment of spermatogonial stem cells derived from CSF-1 receptor knock out mouse.
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