Investigation of molecular indicators related to the degree of pluripotency of iPS cells

2013 Fiscal Year Final Research Report

• Project/Area Number: 24770224
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Nara Women's University (2013); National Institute of Radiological Sciences (2012)
• Principal Investigator: SUGIURA Mayumi 奈良女子大学, 自然科学系, 助教 (60397841)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: iPS / ヒストンエピジェネティクス / 分化能

Research Abstract

Although iPS cells have been generated by various methods, considerable quality variation has been observed. This study aimed at finding the molecular indicators for discriminating the quality of iPSCs. We focused on the histone-epigenetic modifications, and obtained the following results. (1) Acceleration of the histone acetylation was observed only in iPSCs with high developmental potential. (2) TSA-treatment on iPSCs showing low pluripotency elicited a temporary accumulation of histone acetylation, resulting in improving their developmental ability. (3) Analysis of the expression of histone modification-related factors suggested a close correlation between the degree of histone acetylation and the expression level of c-Myc and certain HDAC in iPSCs. Such a close correlation was not observed in ES cells. Gene knockdown experiments showed the HDAC is possible to affect differentiated state of iPSCs. (4) Genome integrity of each iPSC was not direct cause of its developmental potential.

Research Products

• [Journal Article] Induced pluripotent stem cell generation-associated point mutations arise during the initial stages of the conversion of these cells (2014)
  • Author(s): Mayumi Sugiura, Yasuji Kasama, Ryoko Araki, Yuko Hoki, Misato Sunayama, Masahiro Uda, Miki Nakamura, Shunsuke Ando and Masumi Abe
  • Journal Title: Stem Cell Reports Volume: 2(1) Pages: 52-63
  • DOI: 10.1016/j.stemcr.2013.11.006
  • Peer Reviewed
• [Presentation] Acceleration of the histone acetylation in pluripotent iPS cells (2013)
  • Author(s): 杉浦真由美、法喜ゆう子、砂山美里、宇田昌広、荒木良子、安倍真澄
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場、神戸国際展示場、神戸ポートピアホテル(兵庫県神戸市)
  • Year and Date: 20131203-06
• [Presentation] iPS cells generation- associated point mutations (2013)
  • Author(s): 荒木良子、杉浦真由美、笠間康次、砂山美里、宇田昌広、安藤俊輔、中村美樹、法喜ゆう子、安倍真澄
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場、神戸国際展示場、神戸ポートピアホテル(兵庫県神戸市)
  • Year and Date: 20131203-06
• [Presentation] Point mutations in ES cells (2013)
  • Author(s): 砂山美里、杉浦真由美、法喜ゆう子、笠間康次、宇田昌広、中村美樹、安藤俊輔、荒木良子、安倍真澄
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場、神戸国際展示場、神戸ポートピアホテル(兵庫県神戸市)
  • Year and Date: 20131203-06
• [Presentation] Point mutations in ES cells (2013)
  • Author(s): Yuko Hoki, Mayumi Sugiura, Yasuji Kasama, Misato Sunayama, Masahiro Uda, Miki Nakamura, Shunsuke Ando, Ryoko Araki, Masumi Abe
  • Organizer: International Society for Stem Cell Research-ISSCR 11th Annual Meeting
  • Place of Presentation: Boston MA, USA
  • Year and Date: 20130612-15
• [Presentation] iPS cells generation-associated point mutations, International Society for Stem Cell (2013)
  • Author(s): Ryoko Araki, Mayumi Sugiura, Yasuji Kasama, Misato Sunayama, Masahiro Uda, Miki Nakamura, Shunsuke Ando, Yuko Hoki, Masumi Abe
  • Organizer: Research-ISSCR 11th Annual Meeting
  • Place of Presentation: Boston MA, USA
  • Year and Date: 20130612-15
• [Presentation] A Comparison between iPSCs and ESCs reveals that genome reprogramming during iPSC generation causes transversion- predominant point mutations (2012)
  • Author(s): 杉浦真由美、笠間康次、藤森ゆう子、宇田昌広、中村美樹、安藤俊輔、砂山美里、荒木良子、安倍真澄
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡
  • Year and Date: 20121211-14
• [Presentation] A comparison between immunogenicity of iPSCs and of ESCs (2012)
  • Author(s): Ryoko Araki, Masahiro Uda, Yuko Hoki-Fujimori, Miki Nakamura, Shunsuke Ando, Misato Sunayama, Mayumi Sugiura, Hisashi Ideno, Akemi Shimada, Akira Nifuji and Masumi Abe
  • Organizer: International Society for Stem Cell Research-ISSCR 10th Annual Meeting
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 20120613-16
• [Presentation] A Comparison between iPSCs and ESCs reveals reprogramming-associated point mutations (2012)
  • Author(s): Masumi Abe, Mayumi Sugiura, Yasuji Kasama, Yuko Hoki-Fujimori, Masahiro Uda, Miki Nakamura, Shunsuke Ando, Misato Sunayama and Ryoko Araki
  • Organizer: International Society for Stem Cell Research-ISSCR 10th Annual Meeting
  • Place of Presentation: Pacifico Yokohama
  • Year and Date: 2012-06-13