Molecular breeding of next generation super-Agrobacterium

2015 Fiscal Year Final Research Report

• Project/Area Number: 24780001
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Breeding science
• Research Institution: University of Tsukuba
• Principal Investigator: Nonaka Satoko 筑波大学, 生命環境系, 助教 (50580825)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: アグロバクテリウム / transformation / GABA / GAD

Outline of Final Research Achievements

The aim of this study is the increasing of transformation frequency in non-model plants. To increase the transformation frequency, we attempt to improve the Agrobacterium tumefaciens. Plant synthesize the ethylene and GABA during co-cultivation, and these compounds prevent Agrobacterium from transforming T-DNA. Therefore in this study, we try to breeding new Agrobacterium tumefaciens with the ability to reduce GABA. GABA were degraded by GABA transaminase, however the Agrobacterium tumefaciens does not have the enzyme. We isolated the GABA transaminase from Eschelichia coli and introduce the gene using with plasmid. We produced two types of Agrobacterium strains. One was harbouring only GABA transaminase gene, and the other had both acdS and gabT enzymes. The acdS degrade ACC which is precursor of ethylene. Therefore, the strain also reduce ethylene during co-culticvation. These strains are increase the transformation frequency in tomato and erianthus.

Free Research Field

植物育種