2014 Fiscal Year Final Research Report
Generation of the transgenic silkworm having high ability of RNAi
| Project/Area Number |
24780052
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied entomology
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
KOBAYASHI Isao 独立行政法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, 研究員 (70442829)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | カイコ / RNAi / SID-1 |
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| Outline of Final Research Achievements |
To enhance RNAi efficiency in silkworm, we generated the transgenic silkworm expressing SID-1 and tested RNAi efficiency. The silkworm expresses SID-1 under the Gal4/UAS system constantly. In the experiment of Bmwh3-RNAi, it was known that longer dsRNA was effective in gene knockdown. Obvious phenomena were observed in RNAi experiments of POUM2, Varp, BLOS2, og, Bmok, and XDH2. The iap-dsRNA induced high knockdown in SID-1-expressing silkworm.
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| Free Research Field |
分子生物学
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