Analysis of boron-dependent regulatory mechanism of NIP5;1 mRNA degradation in Arabidopsis thaliana

2014 Fiscal Year Final Research Report

• Project/Area Number: 24780057
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant nutrition/Soil science
• Research Institution: The University of Tokyo
• Principal Investigator: TANAKA Mayuki 東京大学, 農学生命科学研究科, 助教 (80546292)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: シロイヌナズナ / ホウ素 / RNAの安定性 / リボソーム / 輸送体 / 5'-非翻訳領域

Outline of Final Research Achievements

Purpose of this study is to understand the regulatory mechanisms of Boron-dependent mRNA degradation of NIP5;1, a boric acid channelin Arabisopsis thaliana.
First, in order to identify genes involved in B-dependent NIP5;1 mRNA degradation, I screened M1 plants from an ethyl methanesulphonate-mutagenized population by GFP fluorescence as an indicator. After screening of M2 plants, five individual mutants were obtained. Second, I revealed that ribosomes stall at the minimum upstream ORF, AUGUAA, in the 5’-untranslated region of NIP5;1, in a boron-dependent manner, and this stalling is accompanied by mRNA degradation. It is possibly that this regulation of gene expression through ribosome stalling at AUGUAA is a general system in eukaryote.

Free Research Field

植物栄養学・土壌学