Isolation and Characterization of STOP1 Ortholog Genes of Tea Plant (Camellia sinensis L.)

2014 Fiscal Year Final Research Report

• Project/Area Number: 24780059
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant nutrition/Soil science
• Research Institution: Shizuoka University
• Principal Investigator: IKKA Takashi 静岡大学, (連合)農学研究科(研究院), 助教 (90580647)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: チャ / アルミニウム耐性 / STOP1転写因子

Outline of Final Research Achievements

Aluminum (Al) and proton (H+) tolerances are essential traits for plants to adapt to acid soil environments. Recently, in Arabidopsis (A. thaliana), it was reported that these tolerances are mediated by a zinc-finger transcription factor, sensitive to proton rhizotoxicity1 (AtSTOP1), which regulates the transcription of multiple genes critical for both tolerances. Tea plants (Camellia sinensis L.) grow well in strongly acidic soils (less than pH 4) containing high levels of Al3+ and H+. However, the mechanisms of both tolerances by tea plants are still poorly clarified. Here, we isolated two STOP1 orthologs (CsSTOP1 and CsSTOP2) from tea plant, and characterized in planta complementation assays. By using CsSTOP1- or CsSTOP2-expressing Arabidopsis transgenic lines, it was suggested that several genes, known to be regulated in the AtSTOP1 under Al3+ and H+ treatments, were also regulated in the CsSTOP1 and CsSTOP2.

Free Research Field

植物栄養学