Sulfur-containing compounds biosynthesis in Bifidobacterium bifidum

2014 Fiscal Year Final Research Report

• Project/Area Number: 24780075
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Kochi University
• Principal Investigator: KATO Shin-ichiro 高知大学, 教育研究部総合科学系, 准教授 (60346707)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 含硫化合物

Outline of Final Research Achievements

Cysteine desulfurase is a PLP-dependent enzyme and generates sulfur atom for the biosynthesis of sulfur-containing compounds, such as thiamine, biotin, and FeS cluster. We used L-[35S]cysteine as a tracer to identify proteins that accept sulfur atom for the biosynthesis of sulfur-containing cofactors in Bifidobacterium adorescentis. We have detected two 35S-labeled proteins by autoradiography. We also focused on the five genes located around cysteine desulfurase gene. Bad0711 and Bad0714 enhanced the cysteine desulfurase activativity and diminished the amount of 35S-label on cysteine desulfurases molecule, respectively. These results suggest that Bad0711 and Bad0714 promote the removal of sulfur on cysteine desulfurases.

Free Research Field

遺伝子工学