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2013 Fiscal Year Final Research Report

Metabolic analysis of a PET degradation bacterium

Research Project

  • PDF
Project/Area Number 24780078
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied microbiology
Research InstitutionKeio University

Principal Investigator

YOSHIDA Shosuke  慶應義塾大学, 理工学部, 助教 (80610766)

Project Period (FY) 2012-04-01 – 2014-03-31
Keywordsポリエチレンテレフタレー ト / ゲノム解析 / トランスクリプトーム解析 / リパーゼ / クチナーゼ / クロロゲン酸エステラーゼ
Research Abstract

In order to elucidate the mechanism that Ideonella sp. No. 201-F6 strain grows on poly(ethylene terephthalate) (PET) as a carbon source, enzymes involved in the PET metabolism were explored. Whole genome analysis of this strain revealed the metabolic potential for various aromatic compounds containing terephthalic acid. Biochemical analyses for selected proteins through the genome/transcriptome information based screening identified two novel enzymes functioning as PET hydrolase and mono(2-hydroxyethyl) terephthalic acid hydrolase, respectively. These discoveries suggested the metabolic route from PET to carbon dioxide and water in this bacterium.

  • Research Products

    (4 results)

All 2014 2013

All Presentation (3 results) (of which Invited: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Presentation] 新規ポリエチレンテレフタレート代謝酵素群の同定と機能解析2014

    • Author(s)
      吉田昭介・捧開維・小田耕平・宮本憲二
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田キャンパス
    • Year and Date
      2014-03-29
  • [Presentation] 難生分解性芳香族ポリマーPETの微生物分解2014

    • Author(s)
      小田耕平・平賀和三・吉田昭介・宮本憲二
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学東山キャンパス
    • Year and Date
      2014-03-29
    • Invited
  • [Presentation] Ideonella sp.No.201-F6株が生産する新規ポリエチレンテレフタレート分解酵素群の同定2013

    • Author(s)
      吉田昭介・捧開維・小田耕平・宮本憲二
    • Organizer
      日本農芸化学会関東支部2013年度支部大会
    • Place of Presentation
      慶應義塾大学日吉キャンパス
    • Year and Date
      2013-11-22
  • [Patent(Industrial Property Rights)] 芳香族ポリエステル分解酵素及び該酵素を用いた芳香族ポリエステル分解方法2013

    • Inventor(s)
      吉田昭介・宮本憲二・小田耕平・木村良晴・平賀和三
    • Industrial Property Rights Holder
      慶應義塾大学
    • Industrial Property Rights Type
      特許特願2013-171745
    • Industrial Property Number
      特願2013-171745
    • Filing Date
      2013-08-21

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Published: 2015-06-25  

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