Study of Y chromosome gene in sex differences of skeletal growth

2013 Fiscal Year Final Research Report

• Project/Area Number: 24780094
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Ehime University
• Principal Investigator: KAZUKI INOUE 愛媛大学, 総合科学研究支援センター, 助教 (60623725)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: Y染色体

Research Abstract

Males have higher bone mass and larger skeletal size than females. Such gender-specific bone phenotype has considered to be generated by the actions of sex steroid hormones. However, an uncharacterized Y chromosome gene is assumed as a significant factor to form gender-specific bone phenotype from clinical observations of patients mutated in the Growth Controlling region on the Y chromosome. To reveal the biological functions of Y chromosome gene in skeletal systems, we generated Y chromosome gene knockout mice by using a novel method. We revealed that Y chromosome gene regulates male skeletal length by controlling cartilage development. Taken together, the present study has uncovered a molecular basis of gender-specific cartilage development.

Research Products

• [Journal Article] Identification of Novel Transcription Factors in Osteoclast Differentiation using Genome–wide Analysis of Open Chromatin Determined by DNase-seq (2014)
  • Author(s): Inoue K and Imai Y
  • Journal Title: J Bone Miner Res
  • DOI: 10.1002/jbmr.2229
  • Peer Reviewed
• [Journal Article] Estrogen receptorαin osteocytes regulates trabecular bone formation in female mice (2014)
  • Author(s): Kondoh S, Inoue K, Igrashi K, Sugizaki, H, Shirode-Fukuda Y, Inoue E, Yu T, Takeuchi J, Kanno J, Bonewald L, Imai Y
  • Journal Title: Bone Volume: 60 Pages: 68-77
  • DOI: 10.1016/j.bone.2013.12.005
  • Peer Reviewed
• [Journal Article] Novel insights into histone modifiers in adipogenesis (2013)
  • Author(s): Okuno Y, Inoue K, Imai Y
  • Journal Title: Adipocyte Volume: 2(4) Pages: 285-8
  • DOI: 10.4161/adip.25731
  • Peer Reviewed
• [Journal Article] Autoimmune regulator, Aire, is a novel regulator of chondrocyte differentiation (2013)
  • Author(s): Si Y, Inoue K, Igarashi K, Kanno J, Imai Y
  • Journal Title: Biochem Biophys Res Commun Volume: 437(4) Pages: 579-84
  • DOI: 10.1016/j.bbrc.2013.07.001
  • Peer Reviewed
• [Journal Article] Female sex hormones ameliorate arthritis in SKG mice (2013)
  • Author(s): Inoue K, Inoue E, Imai Y
  • Journal Title: Biochem Biophys Res Commun Volume: 434(4) Pages: 740-5
  • DOI: 10.1016/j.bbrc.2013.03.111
  • Peer Reviewed
• [Journal Article] Vitamin D receptor in osteoblasts is a negative regulator of bone mass control (2013)
  • Author(s): Yamamoto Y, ... , Inoue K, ....., Imai Y.
  • Journal Title: Endocrinology Volume: 154(3) Pages: 1008-20
  • DOI: 10.1210/en.2012-1542
  • Peer Reviewed
• [Journal Article] Nuclear Receptors in Bone Physiology and Diseases (2012)
  • Author(s): Imai Y, Youn MY, Inoue K, Takada I, Kouzmenko A, Kato S
  • Journal Title: Physiol Rev Volume: 93(2) Pages: 481-523
  • DOI: 10.1152/physrev.00008
  • Peer Reviewed
• [Presentation] Identification of Novel Transcription Factors in Osteoclast differentiation using DNase-seq (2014)
  • Author(s): Kazuki Inoue and Yuuki Imai
  • Organizer: Herbert Fleisch Workshop
  • Place of Presentation: Brugge, Belgium
  • Year and Date: 20140316-18
• [Presentation] Dynamic changes in chromatin accessibility during early osteoclastogenesin (2013)
  • Author(s): Kazuki Inoue and Yuuki Imai
  • Organizer: 2^<nd> Joint Meeting of the International Bone and Mineral Society and the Japan Society for Bone and Mineral Research
  • Place of Presentation: Kobe, Japan
  • Year and Date: 20130528-0601
• [Presentation] Dynamic changes in chromatin accessibility during early osteoclastogenesin (2012)
  • Author(s): Kazuki Inoue and Yuuki Imai
  • Organizer: ASBMR 2012 Annual Meeting
  • Place of Presentation: Minneapolis, USA
  • Year and Date: 2012-10-13