2013 Fiscal Year Final Research Report
Enzymactic synthesis of functional oligosaccharides by nocel carbohydrate phosphorylases
| Project/Area Number |
24780095
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 酵素 / オリゴ糖 |
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| Research Abstract |
Phosphorylases are exolytic enzymes catalyzing phosphorolysis of particular glycosides to produce sugar 1-phosphate with strict substrate specificity. The reaction is reversible, enabling the practical synthesis of oligosaccharides. However, there is little variation among phosphorylases and this limits their utilization for the production of oligosaccharides. Therefore, it would be beneficial to identify phosphorylases with previously unreported substrate specificities. In this study, several bacterial phosphorylase homologues belonging to glycoside hydrolase family 65, 94, and 130 are characterized to be novel phosphorylases showing unreported substrate specificities. In addition, a variety of heterooligosaccharides were synthesized by the reverse phosphorolysis catalyzed by the novel phosphorylases.
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