2013 Fiscal Year Final Research Report
Basic research for protein and lipid production in C1 yeast utilzing autophagy molecules
| Project/Area Number |
24780100
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
OKU Masahide 京都大学, (連合)農学研究科(研究院), 助教 (10511230)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | オートファジー / 液胞 / 脂肪滴 / ペルオキシソーム / メタノール資化性酵母 |
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| Research Abstract |
In this research project, I aimed to obtain insights useful for protein or lipid production in the methylotrophic yeast (C1 yeast), by elucidating molecular functions of autophagy-related (Atg) proteins in this yeast species. As a result, I discovered that 1) Atg8 can act on the morphological regulation of lipid droplets, a storage site of neutral lipids, as revealed by the analysis of several Atg8-fused proteins. And 2) I elucidated the role of two vacuole-localized Atg proteins (Atg18 and Atg21) in peroxisome-targeting autophagy, and also discovered that Atg18 drives the morphological changes of the vacuole in response to various environmental alternations, which is regulated by the phosphorylation status of the protein.
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