2013 Fiscal Year Final Research Report
Construction of novel bio-catalyst based on serine-aminopeptidase
| Project/Area Number |
24780103
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Tottori University |
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Principal Investigator |
ARIMA Jiro 鳥取大学, 農学部, 准教授 (80393411)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | タンパク質工学 / ペプチダーゼ / アミノリシス / ジペプチド / 変異解析 |
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| Research Abstract |
Peptides are attractive for novel biologically active substances. In this study, we attempted construction of a peptide library by chemo-enzymatic synthesis along with generation of bio-catalyst for synthesis of various peptides based on peptidases that catalyze peptide bond formation reaction as a side reaction. We exhaustively selected materials (substrates) for construction of peptide library using peptidases that exhibit peptide bond formation activity. By using the information, we constructed 900 kinds of enzyme reaction mixture library using the reaction of above enzymes. In addition, we found that the change in structure of active site of above enzymes by genetic engineering techniques exhibited increase of effectiveness for peptide bond formation.
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